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Part:BBa_M36995:Design

Designed by: Nathan Barnett, Meghan Bowler, Kian Torabian   Group: Stanford BIOE44 - S11   (2011-04-28)


Type II Secreted Antifreeze Actuator in E. coli (5-Repeat)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 109
    Illegal PstI site found at 164
    Illegal PstI site found at 191
    Illegal PstI site found at 209
    Illegal PstI site found at 221
    Illegal PstI site found at 257
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 109
    Illegal PstI site found at 164
    Illegal PstI site found at 191
    Illegal PstI site found at 209
    Illegal PstI site found at 221
    Illegal PstI site found at 257
    Illegal NotI site found at 292
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 321
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 109
    Illegal PstI site found at 164
    Illegal PstI site found at 191
    Illegal PstI site found at 209
    Illegal PstI site found at 221
    Illegal PstI site found at 257
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 109
    Illegal PstI site found at 164
    Illegal PstI site found at 191
    Illegal PstI site found at 209
    Illegal PstI site found at 221
    Illegal PstI site found at 257
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This uses the 5-Repeat segments of the antifreeze protein, which can be fluctuated and remain functioning. Studies have shown, however, that increasing the number of repeating units in the antifreeze protein boosts its ability to act as antifreeze.


Source

Based on a protein from the white flounder, Pseudopleuronecta americanus.

References